Isolation and Identification of Staphylococcus aurous in Packaged Milk
Abstract
Milk serves as a good nutrient medium for the growth of many types of microorganisms. Therefore, it can get dirty during milking, transport, and storage. The bad germs (pathogens) in milk and milk products which are most common and dangerous are Staphylococcus aureus since they make special poisons (enterotoxins) that hurt people when eaten. Aims: Detection of Staphylococcus aurous bacteria in milk sold in Iraqi markets for the purpose of quality and safety of canned milk. Methods: This work tries to find the Staphylococcus aureus germ from milk samples packaged milk sold in markets of Karbala, Diyala, Basra Wasit governorates between October 2021 and March 2022. Fifty samples were taken and put on a special plate called Mannitol Salt Agar then kept at 37°C for up to 48 hours. Bacteria grew only on six plates; one of them- number 21 had with 74 colonies the highest count. Results: The results indicate a reduction in the standards of hygiene during milking and handling operations, basically reflecting unhealthy practices. This serves to bring out an invisible challenge in our local markets; hence these findings are very relevant to public health decision-makers. It should strengthen their oversight of the milk production as well as distribution chains for consumer safety.
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References
L. M. Parsons, Á. Somoskövi, C. Gutierrez, E. Lee, C. N. Paramasivan, A. L. Abimiku et al., "Laboratory diagnosis of tuberculosis in resource-poor countries: challenges and opportunities," Clin. Microbiol. Rev., vol. 24, no. 2, pp. 314–350, 2011
S. P. Oliver, B. M. Jayarao, and R. A. Almeida, "Foodborne pathogens in milk and the dairy farm environment: food safety and public health implications," Foodborne Pathog. Dis., vol. 2, no. 2, pp. 115–129, 2005.
World Health Organization, WHO estimates of the global burden of foodborne diseases. Geneva, Switzerland: WHO, 2015.
D. Morgan, C. P. Newman, D. N. Hutchinson, A. M. Walker, B. Rowe, and F. Majid, "Verotoxin producing Escherichia coli O157 infections associated with the consumption of yoghurt," Epidemiol. Infect., vol. 111, no. 2, pp. 181–188, 1993.
M. Pal, S. Mulu, M. Tekle, S. V. Pintoo, and J. Prajapati, "Bacterial contamination of dairy products," Beverage Food World, vol. 43, no. 9, pp. 40–43, 2016.
J. A. Painter, R. M. Hoekstra, T. Ayers, R. V. Tauxe, C. R. Braden, F. J. Angulo, and P. M. Griffin, "Attribution of foodborne illnesses, hospitalizations, and deaths to food commodities by using outbreak data, United States, 1998–2008," Emerg. Infect. Dis., vol. 19, no. 3, p. 407, 2013.
M. Acco, F. S. Ferreira, J. A. P. Henriques, and E. C. Tondo, "Identification of multiple strains of Staphylococcus aureus colonizing nasal mucosa of food handlers," Food Microbiol., vol. 20, no. 5, pp. 489–493, 2003.
A. B. Pebdeni, M. Hosseini, and A. Barkhordari, "Smart fluorescence aptasensor using nanofiber functionalized with carbon quantum dot for specific detection of pathogenic bacteria in the wound," Talanta, vol. 246, p. 123454, 2022.
K. Smith, K. Peter, D. Hanel, and S. Melchior, "Food borne pathogenic microorganisms and natural toxins," Food Drug Adm. Center Food Saf. Appl. Nutr., vol. 10, pp. 119–150, 2007.
L. M. El-Malt, K. A. Hameed, and A. S. Mohammed, "Microbiological evaluation of yoghurt products in Qena city, Egypt," 2013.
P. Moroni, G. Pisoni, C. Vimercati, M. Rinaldi, B. Castiglioni, P. Cremonesi, and P. Boettcher, "Characterization of Staphylococcus aureus isolated from chronically infected dairy goats," J. Dairy Sci., vol. 88, no. 10, pp. 3500–3509, 2005.
D. Bergonier, R. De Crémoux, R. Rupp, G. Lagriffoul, and X. Berthelot, "Mastitis of dairy small ruminants," Vet. Res., vol. 34, no. 5, pp. 689–716, 2003.
A. Zecconi and G. Hahn, "Staphylococcus aureus in raw milk and human health risk," 1999.
A. M. Bhutto, R. A. Soomro, S. Nonaka, and Y. Hashiguchi, "Detection of new endemic areas of cutaneous leishmaniasis in Pakistan: a 6-year study," Int. J. Dermatol., vol. 42, no. 7, pp. 543–548, 2003.
K. McMillan, S. C. Moore, C. M. McAuley, N. Fegan, and E. M. Fox, "Characterization of Staphylococcus aureus isolates from raw milk sources in Victoria, Australia," BMC Microbiol., vol. 16, pp. 1–12, 2016.
H. J. Jørgensen, T. Mørk, and L. M. Rørvik, "The occurrence of Staphylococcus aureus on a farm with small-scale production of raw milk cheese," J. Dairy Sci., vol. 88, no. 11, pp. 3810–3817, 2005.
D. Daka, S. G. Silassie, and D. Yihdego, "Antibiotic-resistance Staphylococcus aureus isolated from cow’s milk in the Hawassa area, South Ethiopia," Ann. Clin. Microbiol. Antimicrob., vol. 11, pp. 1–6, 2012.
Central Organization for Standardization and Quality Control, Iraqi standard No. 2270/5: Microbial limits in food – Milk and its products (in Arabic), Republic of Iraq, Ministry of Planning, 2022.
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