Liver Histological Effects Induced by Frying Fodder with Olive Oil, Bay Leaves and Ginger Roots on Healthy Mice

Rahman J.  Younis; Abdullah M. Ajil; Irfan W.  Mahmood

doi:10.51699/cajotas.v7i2.1673

Authors

Rahman J. Younis Department of Science, College of Basic Education/Shirqat, Tikrit University, 34005, Salah Al Deen, Iraq
Abdullah M. Ajil Department of Dairy Science and Technology, College of Food Sciences Al-Shirqat, 34005, Salah Al Deen, Tikrit University
Irfan W. Mahmood Department of Science, College of Basic Education/Shirqat, Tikrit University, 34005, Salah Al Deen, Iraq

DOI:

https://doi.org/10.51699/cajotas.v7i2.1673

Keywords:

Ginger Roots, Bay Leaves, Olive Oil, Liver

Abstract

Humans have studied the importance of plants and their oils, classifying them as beneficial or harmful, and have used healthy ones to treat diseases, as most published research discuses the benefits of oils when they are fresh. Therefore, the current study aimed to evaluate the histological effects of certain oils when fried and to investigate the potential protective effects of medicinal plants possessing detoxifying properties. These oils are widely used in cooking, as they are generally considered healthy and harmless to the body. Male and female mice were divided into four groups: a control group, food that was fried in olive oil, Boil the food in olive oil with ginger and bay leaves separately. The injection was administered intravenously in a concentrated form, resulting in the deterioration of many liver cells, localized necrosis in others, nuclear pyknosis, and dilatation of blood sinusoids were seen in the group fed meals fried in olive oil, and a size and number increasing of liver macrophages. Severe hyperemia in the portal vein, fibrin deposition on one side of its lumen, and Additionally, there was a substantial infiltration of inflammatory cells. On the other hand, the group that was given food that was fried in olive oil with bay leaves and the group that was fed food that was fried in olive oil with ginger roots both had almost normal histological structure.

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References

A. Zeb, “Chemistry and liquid chromatography methods for the analyses of primary oxidation products of triacylglycerols,” Free Radical Research, vol. 49, no. 5, pp. 549–564, 2015, doi: 10.3109/10715762.2015.1022540.

I. Staprans, D. A. Hardman, X. M. Pan, and K. R. Feingold, “Effect of oxidized lipids in the diet on oxidized lipid levels in postprandial serum chylomicrons of diabetic patients,” Diabetes Care, vol. 22, no. 2, pp. 300–306, 1999, doi: 10.2337/diacare.22.2.300.

A. Tiwari, A. Shukla, and P. Kumar Samal, “Evaluation of anti-hyperlipidemic and anti-atherogenic activity of asiatic acid and its effect on lipid peroxidation in hyperlipidemic rats,” Journal of Biochemical and Molecular Toxicology, vol. 39, no. 5, p. e70255, 2025, doi: 10.1002/jbt.70255.

A. Zeb and A. A. Khan, “Improvement of serum biochemical parameters and hematological indices through α-tocopherol administration in dietary oxidized olive oil induced toxicity in rats,” Frontiers in Nutrition, vol. 5, p. 137, 2019, doi: 10.3389/fnut.2018.00137.

P. K. Nayak, U. M. A. Dash, K. Rayaguru, and K. R. Krishnan, “Physio-chemical changes during repeated frying of cooked oil: A review,” Journal of Food Biochemistry, vol. 40, no. 3, pp. 371–390, 2016, doi: 10.1111/jfbc.12215.

C. S. Santos, L. Molina-Garcia, S. C. Cunha, and S. Casal, “Fried potatoes: Impact of prolonged frying in monounsaturated oils,” Food Chemistry, vol. 243, pp. 192–201, 2018, doi: 10.1016/j.foodchem.2017.09.117.

R. Ambra, S. Lucchetti, and G. Pastore, “A review of the effects of olive oil-cooking on phenolic compounds,” Molecules, vol. 27, no. 3, p. 661, 2022, doi: 10.3390/molecules27030661.

C. Jimenez-Lopez et al., “Bioactive compounds and quality of extra virgin olive oil,” Foods, vol. 9, no. 8, p. 1014, 2020, doi: 10.3390/foods9081014.

S. Batool, R. A. Khera, M. A. Hanif, and M. A. Ayub, “Bay leaf,” in Medicinal Plants of South Asia, pp. 63–74, 2020, doi: 10.1016/B978-0-08-102659-5.00005-7.

P. Ballester, B. Cerdá, R. Arcusa, J. Marhuenda, K. Yamedjeu, and P. Zafrilla, “Effect of ginger on inflammatory diseases,” Molecules, vol. 27, no. 21, p. 7223, 2022, doi: 10.3390/molecules27217223.

L. G. Luna, Manual of Histologic Staining Methods of the Armed Forces Institute of Pathology. New York: McGraw-Hill, 1968.

S. Casal, R. Malheiro, A. Sendas, B. P. Oliveira, and J. A. Pereira, “Olive oil stability under deep-frying conditions,” Food and Chemical Toxicology, vol. 48, no. 10, pp. 2972–2979, 2010, doi: 10.1016/j.fct.2010.07.036.

C. S. Santos, R. Cruz, S. C. Cunha, and S. Casal, “Effect of cooking on olive oil quality attributes,” Food Research International, vol. 54, no. 2, pp. 2016–2024, 2013, doi: 10.1016/j.foodres.2013.04.014.

D. Zhao et al., “Oxidative stability of phytosterols in camellia seed oil during heating: The impact of different antioxidants,” Foods, vol. 14, no. 13, p. 2297, 2025, doi: 10.3390/foods14132297.

M. M. Bakr, H. M. Taher, F. K. Hussein, and A. H. Mohamed, “Study the effective role of metronidazole nanoemulsion for the treatment of skin lesions in mice induced by Entamoeba histolytica,” South Asian Research Journal of Biology and Applied Biosciences, vol. 6, no. 1, pp. 1–7, 2024, doi: 10.36346/sarjbab.2024.v06i01.001.

A. Sharma, J. Singh, and S. Kumar, “Bay leaves,” in Handbook of Herbs and Spices, pp. 73–85, 2012, doi: 10.1533/9780857095671.73.

K. Singletary, “Bay leaf: Potential health benefits,” Nutrition Today, vol. 56, no. 4, pp. 202–208, 2021, doi: 10.1097/NT.0000000000000493.

C. Nutakor, J. A. Essiedu, P. Adadi, and O. N. Kanwugu, “Ginger beer: An overview of health benefits and recent developments,” Fermentation, vol. 6, no. 4, p. 102, 2020, doi: 10.3390/fermentation6040102.

M. Zhang et al., “Ginger (Zingiber officinale Rosc.) and its bioactive components are potential resources for health beneficial agents,” Phytotherapy Research, vol. 35, no. 2, pp. 711–742, 2021, doi: 10.1002/ptr.6858.

R. H. Ali, F. K. Hussein, D. M. Nasar, and A. Abd Al Salam Salem, “The relevance of mitochondrial DNA mutation in human diseases and forensic sciences,” Al-Nahrain Journal of Science, vol. 28, no. 1, pp. 96–106, 2025, doi: 10.22401/ANJS.28.1.11.

I. Bischoff-Kont and R. Fürst, “Benefits of ginger and its constituent 6-shogaol in inhibiting inflammatory processes,” Pharmaceuticals, vol. 14, no. 6, p. 571, 2021, doi: 10.3390/ph14060571.

S. R. Hasan, F. M. Junaid, B. M. Mahdi, and F. K. Hussein, “Therapeutic applications of medicinal plants for the treatment of human intestinal diarrhea: Review article,” South Asian Journal of Life Sciences, vol. 13, pp. 20–24, 2025, doi: 10.17582/journal.sajls/2025/13.20.24.

R. H. Ma et al., “A recent update on the multifaceted health benefits associated with ginger and its bioactive components,” Food & Function, vol. 12, no. 2, pp. 519–542, 2021, doi: 10.1039/D0FO02834G.