Biodynamic and Thermodynamic Study of Alanine Aminopeptide (AAP) Partially Purified from the Urine of Patients with Chronic Renal Injury

Jasim Mohammed Thahir

doi:10.51699/cajotas.v7i1.1657

Authors

Jasim Mohammed Thahir Ministry of Education – Open Education College

DOI:

https://doi.org/10.51699/cajotas.v7i1.1657

Keywords:

Alanine Aminopeptidase (AAP), Renal Failure, Urinary Biomarker, Enzyme Kinetics, Thermodynamic Parameters

Abstract

Alanine aminotransferase (AAP) activity was measured in the urine of healthy individuals, with an activity level of 7.49 ≤ 2.76 IU/L. In infected individuals, the activity level was 24.83 ≤ 9.66 IU/L. The results showed elevated AAP activity in the urine of 97% of patients compared to healthy individuals. AAP was purified from the urine of patients with renal failure using gel filtration chromatography with Sephadex G-50 filtration gel. AAP was separated from the urine of patients with renal failure using ion-exchange chromatography with DEAE-Sephadex A-50 resin. It was observed that the isoform obeys the Arrhenius equation up to 37 m⁻¹, and Ea and Q10 were determined. A thermodynamic study was performed on the binding of the substrate (alanine-4-naturoanilide) to the alanine aminopeptide (AAP) isoforms. Using the van't Hoff and Arrhenius equations, the thermodynamic coefficients for the standard state (ΔSO, ΔGO, ΔHO) and the transition state (ΔS*, ΔG*, ΔH*) were determined.

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